AIDA Phase 1 Data Freeze v2: Chinese, Indian, Japanese, Korean, Malay, and Thai donors in Japan, Singapore, South Korea, Thailand, and India

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Cell Metadata

CD14+_Monocyte

Ontology Term: CD14-positive monocyteCL:0001054
SynonymsTerms that have the same, or nearly the same, meaning as the selected cell type.: monocyte, classical monocyte
CategoryClass of which the cell type is a subclass.: monocyteCL:0000576
Marker Gene EvidenceGene markers used by researcher to assign this cell annotation.: CD68, CD14, CSF3R, S100A12, S100A8, S100A9
Canonical Marker Genes: unknown
Rationale: Myeloid and pDC cells were identified via RCAv2. Integration and sub-clustering of all myeloid and pDC cells were performed, and these clusters were manually annotated based on expression of CD14, and after examination of FCGR3A (CD16) expression.

Set of Labels Description: These author cell type labels were assigned largely at the per-cluster level. We identified broad cell types using RCAv2-based clustering (B, CD34+ haematopoietic stem and progenitor cell (HSPC), myeloid, natural killer (NK), plasma cell, plasmacytoid dendritic cell (pDC), platelet, and T). We then performed cell population-specific (B; myeloid + pDC; CD4+ T and dnT; non-CD4+ T and NK and ILC) integration (Seurat RPCA) and sub-clustering (Louvain clustering), and annotated clusters based on their marker genes.
Annotation Method: manual

Ontology Term: CD14-positive monocyteCL:0001054
SynonymsTerms that have the same, or nearly the same, meaning as the selected cell type.: monocyte, classical monocyte
CategoryClass of which the cell type is a subclass.: monocyteCL:0000576
Marker Gene EvidenceGene markers used by researcher to assign this cell annotation.: CD68, CD14, CSF3R, S100A12, S100A8, S100A9
Canonical Marker Genes: unknown
Rationale: Myeloid and pDC cells were identified via RCAv2. Integration and sub-clustering of all myeloid and pDC cells were performed, and these clusters were manually annotated based on expression of CD14, and after examination of FCGR3A (CD16) expression.

Set of Labels Description: These author cell type labels were assigned largely at the per-cluster level. We identified broad cell types using RCAv2-based clustering (B, CD34+ haematopoietic stem and progenitor cell (HSPC), myeloid, natural killer (NK), plasma cell, plasmacytoid dendritic cell (pDC), platelet, and T). We then performed cell population-specific (B; myeloid + pDC; CD4+ T and dnT; non-CD4+ T and NK and ILC) integration (Seurat RPCA) and sub-clustering (Louvain clustering), and annotated clusters based on their marker genes.
Annotation Method: manual