AIDA Phase 1 Data Freeze v2: Chinese, Indian, Japanese, Korean, Malay, and Thai donors in Japan, Singapore, South Korea, Thailand, and India

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Cell Metadata

CD16+_Monocyte

Ontology Term: CD14-low, CD16-positive monocyteCL:0002396
SynonymsTerms that have the same, or nearly the same, meaning as the selected cell type.: non-classical monocyte, human
CategoryClass of which the cell type is a subclass.: monocyteCL:0000576
Marker Gene EvidenceGene markers used by researcher to assign this cell annotation.: CD68, FCGR3A, CX3CR1, CDKN1C, CSF1R, ITGAL
Canonical Marker Genes: unknown
Rationale: Myeloid and pDC cells were identified via RCAv2. Integration and sub-clustering of all myeloid and pDC cells were performed, and these clusters were manually annotated based on expression of FCGR3A (CD16) and other marker genes. This annotation includes clusters that may contain intermediate monocytes.

Set of Labels Description: These author cell type labels were assigned largely at the per-cluster level. We identified broad cell types using RCAv2-based clustering (B, CD34+ haematopoietic stem and progenitor cell (HSPC), myeloid, natural killer (NK), plasma cell, plasmacytoid dendritic cell (pDC), platelet, and T). We then performed cell population-specific (B; myeloid + pDC; CD4+ T and dnT; non-CD4+ T and NK and ILC) integration (Seurat RPCA) and sub-clustering (Louvain clustering), and annotated clusters based on their marker genes.
Annotation Method: manual

Ontology Term: CD14-low, CD16-positive monocyteCL:0002396
SynonymsTerms that have the same, or nearly the same, meaning as the selected cell type.: non-classical monocyte, human
CategoryClass of which the cell type is a subclass.: monocyteCL:0000576
Marker Gene EvidenceGene markers used by researcher to assign this cell annotation.: CD68, FCGR3A, CX3CR1, CDKN1C, CSF1R, ITGAL
Canonical Marker Genes: unknown
Rationale: Myeloid and pDC cells were identified via RCAv2. Integration and sub-clustering of all myeloid and pDC cells were performed, and these clusters were manually annotated based on expression of FCGR3A (CD16) and other marker genes. This annotation includes clusters that may contain intermediate monocytes.

Set of Labels Description: These author cell type labels were assigned largely at the per-cluster level. We identified broad cell types using RCAv2-based clustering (B, CD34+ haematopoietic stem and progenitor cell (HSPC), myeloid, natural killer (NK), plasma cell, plasmacytoid dendritic cell (pDC), platelet, and T). We then performed cell population-specific (B; myeloid + pDC; CD4+ T and dnT; non-CD4+ T and NK and ILC) integration (Seurat RPCA) and sub-clustering (Louvain clustering), and annotated clusters based on their marker genes.
Annotation Method: manual