Concerted changes in the pediatric single-cell intestinal ecosystem before and after anti-TNF blockade

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Cell Metadata

T

Ontology Term: T cellCL:0000084
SynonymsTerms that have the same, or nearly the same, meaning as the selected cell type.: T-lymphocyte, immature T cell, T-cell, mature T cell, T lymphocyte
CategoryClass of which the cell type is a subclass.: T cellCL:0000084
Marker Gene EvidenceGene markers used by researcher to assign this cell annotation.: CD3E, TRAC, CD3D
Canonical Marker Genes: TRAC, CD3
Rationale: T cells express the subunits of T cell receptor proteins - TRAC (the constant region) and CD3. T cells are a large, heterogeneous group here, and we haven't annotated subclusters except for with heuristic names for within-T marker genes (e.g. T.FOXP3.MKI67), so markers for T here are broad.

Set of Labels Description: In this paper we used an automated iterative subclustering algorithm to find hundreds of cell state clusters, after which we merged unsupported clusters and annotated each resulting cluster with a better-known cell subtype. The high-res cell state clusters are annotated as subtype.gene1.gene2, so in some cases we didn't directly name some commonly named subtypes, such as pericytes. When we run DE on the resulting subtypes, we find many canonical and scRNA-suggested marker genes.
Annotation Method: both
Algorithm Name: ARBOL
Algorithm Version: v1.0
Algorithm URL: https://github.com/jo-m-lab/ARBOL/

Ontology Term: T cellCL:0000084
SynonymsTerms that have the same, or nearly the same, meaning as the selected cell type.: T-lymphocyte, immature T cell, T-cell, mature T cell, T lymphocyte
CategoryClass of which the cell type is a subclass.: T cellCL:0000084
Marker Gene EvidenceGene markers used by researcher to assign this cell annotation.: CD3E, TRAC, CD3D
Canonical Marker Genes: TRAC, CD3
Rationale: T cells express the subunits of T cell receptor proteins - TRAC (the constant region) and CD3. T cells are a large, heterogeneous group here, and we haven't annotated subclusters except for with heuristic names for within-T marker genes (e.g. T.FOXP3.MKI67), so markers for T here are broad.

Set of Labels Description: In this paper we used an automated iterative subclustering algorithm to find hundreds of cell state clusters, after which we merged unsupported clusters and annotated each resulting cluster with a better-known cell subtype. The high-res cell state clusters are annotated as subtype.gene1.gene2, so in some cases we didn't directly name some commonly named subtypes, such as pericytes. When we run DE on the resulting subtypes, we find many canonical and scRNA-suggested marker genes.
Annotation Method: both
Algorithm Name: ARBOL
Algorithm Version: v1.0
Algorithm URL: https://github.com/jo-m-lab/ARBOL/