Concerted changes in the pediatric single-cell intestinal ecosystem before and after anti-TNF blockade

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Cell Metadata

Tuft

Ontology Term: intestinal tuft cellCL:0019032
SynonymsTerms that have the same, or nearly the same, meaning as the selected cell type.: intestinal brush cell, tuft cell
CategoryClass of which the cell type is a subclass.: epithelial cellCL:0000066
Marker Gene EvidenceGene markers used by researcher to assign this cell annotation.: KRT8, KRT18, LRMP, SH2D6, SPIB
Canonical Marker Genes: SPIB
Rationale: Keratin expressing epithelial cells that share some markers for secretory cells such as MARCKSL1 but also display canonical and newly suggested markers for tuft cells, SPIB, SH2D6, and LRMP.
Rationale DOI: 10.1038/s41467-021-23587-x 10.1016/j.immuni.2022.03.001

Set of Labels Description: In this paper we used an automated iterative subclustering algorithm to find hundreds of cell state clusters, after which we merged unsupported clusters and annotated each resulting cluster with a better-known cell subtype. The high-res cell state clusters are annotated as subtype.gene1.gene2, so in some cases we didn't directly name some commonly named subtypes, such as pericytes. When we run DE on the resulting subtypes, we find many canonical and scRNA-suggested marker genes.
Annotation Method: both
Algorithm Name: ARBOL
Algorithm Version: v1.0
Algorithm URL: https://github.com/jo-m-lab/ARBOL/

Ontology Term: intestinal tuft cellCL:0019032
SynonymsTerms that have the same, or nearly the same, meaning as the selected cell type.: intestinal brush cell, tuft cell
CategoryClass of which the cell type is a subclass.: epithelial cellCL:0000066
Marker Gene EvidenceGene markers used by researcher to assign this cell annotation.: KRT8, KRT18, LRMP, SH2D6, SPIB
Canonical Marker Genes: SPIB
Rationale: Keratin expressing epithelial cells that share some markers for secretory cells such as MARCKSL1 but also display canonical and newly suggested markers for tuft cells, SPIB, SH2D6, and LRMP.
Rationale DOI: 10.1038/s41467-021-23587-x 10.1016/j.immuni.2022.03.001

Set of Labels Description: In this paper we used an automated iterative subclustering algorithm to find hundreds of cell state clusters, after which we merged unsupported clusters and annotated each resulting cluster with a better-known cell subtype. The high-res cell state clusters are annotated as subtype.gene1.gene2, so in some cases we didn't directly name some commonly named subtypes, such as pericytes. When we run DE on the resulting subtypes, we find many canonical and scRNA-suggested marker genes.
Annotation Method: both
Algorithm Name: ARBOL
Algorithm Version: v1.0
Algorithm URL: https://github.com/jo-m-lab/ARBOL/