Concerted changes in the pediatric single-cell intestinal ecosystem before and after anti-TNF blockade

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Cell Metadata

Secretory

Ontology Term: secretory cellCL:0000151
Synonyms Terms that have the same, or nearly the same, meaning as the selected cell type.: unknown
Category Class of which the cell type is a subclass.: epithelial cellCL:0000066
Ontology Assessment Disagreements with any aspect of the Cell Ontology.: —
Marker Gene Evidence Gene markers used by researcher to assign this cell annotation.: REG1A, REG1B, LCN2, SPINK1, REG3G, KRT18
Canonical Marker Genes: ALPI, MUC2, TFF3, CHGA, SYP, LYZ, DEFA5
Rationale: These are epithelial cells based. onKRT18 expression and they are not clearly fitting into goblet, EEC, paneth, or other specific secretory cell subsets, but they are high in SPINK1, REG1A/B, and LCN2. LCN2 and SPINK1 are secreted enzymes

Set of Labels Description: In this paper we used an automated iterative subclustering algorithm to find hundreds of cell state clusters, after which we merged unsupported clusters and annotated each resulting cluster with a better-known cell subtype. The high-res cell state clusters are annotated as subtype.gene1.gene2, so in some cases we didn't directly name some commonly named subtypes, such as pericytes. When we run DE on the resulting subtypes, we find many canonical and scRNA-suggested marker genes.
Annotation Method: both
Algorithm Name: ARBOL
Algorithm Version: v1.0
Algorithm URL: https://github.com/jo-m-lab/ARBOL/

Ontology Term: secretory cellCL:0000151
Synonyms Terms that have the same, or nearly the same, meaning as the selected cell type.: unknown
Category Class of which the cell type is a subclass.: epithelial cellCL:0000066
Ontology Assessment Disagreements with any aspect of the Cell Ontology.: —
Marker Gene Evidence Gene markers used by researcher to assign this cell annotation.: REG1A, REG1B, LCN2, SPINK1, REG3G, KRT18
Canonical Marker Genes: ALPI, MUC2, TFF3, CHGA, SYP, LYZ, DEFA5
Rationale: These are epithelial cells based. onKRT18 expression and they are not clearly fitting into goblet, EEC, paneth, or other specific secretory cell subsets, but they are high in SPINK1, REG1A/B, and LCN2. LCN2 and SPINK1 are secreted enzymes

Set of Labels Description: In this paper we used an automated iterative subclustering algorithm to find hundreds of cell state clusters, after which we merged unsupported clusters and annotated each resulting cluster with a better-known cell subtype. The high-res cell state clusters are annotated as subtype.gene1.gene2, so in some cases we didn't directly name some commonly named subtypes, such as pericytes. When we run DE on the resulting subtypes, we find many canonical and scRNA-suggested marker genes.
Annotation Method: both
Algorithm Name: ARBOL
Algorithm Version: v1.0
Algorithm URL: https://github.com/jo-m-lab/ARBOL/