An Atlas of Cells in the Human Tonsil

Main

Cell Metadata

Mast

Ontology Term: mast cellCL:0000097
SynonymsTerms that have the same, or nearly the same, meaning as the selected cell type.: tissue basophil, histaminocyte, mastocyte, labrocyte
CategoryClass of which the cell type is a subclass.: mast cellCL:0000097
Marker Gene EvidenceGene markers used by researcher to assign this cell annotation.: TPSAB1, TPSB2, MS4A2
Canonical Marker Genes: TPSAB1, TPSB2, MS4A2
Rationale: This cluster has expressions of classical markers for Mast cells KIT, TPSAB1 and TPSB2. Top markers of this cluster were: TPSAB1, TPSB2, CPA3, MS4A2, LTC4S, HPGD, HPGDS and LMO4.
Rationale DOI: 10.1182/bloodadvances.2022006969

Set of Labels Description: The annotation provided in this first version comprises 42 categories that provide a stable categories to classify single-cell transcriptomes of SLOs, useful for tools like Azimuth (see external URLs). In the next version, we will add a more detailed classification, encompassing all cell types and states identified in the tonsil atlas. A validation cohort was included to confirm the presence and accuracy of each annotation this latter level, using criteria such as cell neighborhood preservation, conservation of bona fide marker genes, and annotation confidence derived from the KNN classifier. For a full description and interpretation of this validation cohort, please refer to the final section of the manuscript.
Annotation Method: manual

Ontology Term: mast cellCL:0000097
SynonymsTerms that have the same, or nearly the same, meaning as the selected cell type.: tissue basophil, histaminocyte, mastocyte, labrocyte
CategoryClass of which the cell type is a subclass.: mast cellCL:0000097
Marker Gene EvidenceGene markers used by researcher to assign this cell annotation.: TPSAB1, TPSB2, MS4A2
Canonical Marker Genes: TPSAB1, TPSB2, MS4A2
Rationale: This cluster has expressions of classical markers for Mast cells KIT, TPSAB1 and TPSB2. Top markers of this cluster were: TPSAB1, TPSB2, CPA3, MS4A2, LTC4S, HPGD, HPGDS and LMO4.
Rationale DOI: 10.1182/bloodadvances.2022006969

Set of Labels Description: The annotation provided in this first version comprises 42 categories that provide a stable categories to classify single-cell transcriptomes of SLOs, useful for tools like Azimuth (see external URLs). In the next version, we will add a more detailed classification, encompassing all cell types and states identified in the tonsil atlas. A validation cohort was included to confirm the presence and accuracy of each annotation this latter level, using criteria such as cell neighborhood preservation, conservation of bona fide marker genes, and annotation confidence derived from the KNN classifier. For a full description and interpretation of this validation cohort, please refer to the final section of the manuscript.
Annotation Method: manual