Skin fibroblast scRNA-seq atlas in health

Main

Cell Metadata

F2: Universal

Full Name: Universal fibroblast
Closest Ontology Term: fibroblastCL:0000057
Synonyms Terms that have the same, or nearly the same, meaning as the selected cell type.: Steady-state like fibroblast, Reticular fibroblast
Category Class of which the cell type is a subclass.: stromal cellCL:0000499
Ontology Assessment Disagreements with any aspect of the Cell Ontology.: —
Marker Gene Evidence Gene markers used by researcher to assign this cell annotation.: CD34, PI16, MFAP5, DPP4, PCOLCE2, LGR5, SLPI, CD70, CTHRC1
Canonical Marker Genes: CD34, PI16, MFAP5
Rationale: We named this population based in highest expression of CD34, PI16, and MFAP5, which are reported as UniversalPi16+ fibroblast markers in Buechler et al. This population also had the highest expression of LGR5, in agreement with a potential stem state reported by Buechler. Universal fibroblasts have been reported in the stroma in skin (see McCartney et al. 2024 (in DOI links)). Our cross-tissue analysis also supported these fibroblasts as Universal. However, we note that F2/3: Perivascular fibroblasts also have universal features.
Rationale DOI: 10.1038/s41586-021-03549-5.10.12688/f1000research.143511.1.

Set of Labels Description: Aim to harmonise skin fibroblast labels across studies in both health and disease. This specific dataset includes only healthy/non-lesional cells.
Annotation Method: manual

Full Name: Universal fibroblast
Closest Ontology Term: fibroblastCL:0000057
Synonyms Terms that have the same, or nearly the same, meaning as the selected cell type.: Steady-state like fibroblast, Reticular fibroblast
Category Class of which the cell type is a subclass.: stromal cellCL:0000499
Ontology Assessment Disagreements with any aspect of the Cell Ontology.: —
Marker Gene Evidence Gene markers used by researcher to assign this cell annotation.: CD34, PI16, MFAP5, DPP4, PCOLCE2, LGR5, SLPI, CD70, CTHRC1
Canonical Marker Genes: CD34, PI16, MFAP5
Rationale: We named this population based in highest expression of CD34, PI16, and MFAP5, which are reported as UniversalPi16+ fibroblast markers in Buechler et al. This population also had the highest expression of LGR5, in agreement with a potential stem state reported by Buechler. Universal fibroblasts have been reported in the stroma in skin (see McCartney et al. 2024 (in DOI links)). Our cross-tissue analysis also supported these fibroblasts as Universal. However, we note that F2/3: Perivascular fibroblasts also have universal features.
Rationale DOI: 10.1038/s41586-021-03549-5.10.12688/f1000research.143511.1.

Set of Labels Description: Aim to harmonise skin fibroblast labels across studies in both health and disease. This specific dataset includes only healthy/non-lesional cells.
Annotation Method: manual