Human lymph nodes with partial T and B cell depletion

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Cell Metadata

CD16hi_NK_cells

Ontology Term: mature natural killer cellCL:0000824
Synonyms Terms that have the same, or nearly the same, meaning as the selected cell type.: unknown
Category Class of which the cell type is a subclass.: natural killer cellCL:0000623
Ontology Assessment Disagreements with any aspect of the Cell Ontology.: —
Marker Gene Evidence Gene markers used by researcher to assign this cell annotation.: NCAM1, FCGR3A, KRLF1, NKG7, EOMES, KLRB1
Canonical Marker Genes: NCAM1, FCGR3A, EOMES
Rationale: This cell type was annotated as a natural killer cell by canonical gene expression, but displayed higher CD16 expression that the other natural killer cell subset within the dataset. Slightly lower transcript levels of NCAM1 may suggest ontology term CL:0000939 would be more appropriate however, flow cytometry staining did not indicate differing levels of CD56 at protein level. Standard accepted NK subsets, and described in tonsil Azimuth dataset
Rationale DOI: 10.1016/j.immuni.2024.01.006

Set of Labels Description: Cell annotations were based on the finest possible label that could describe the 16 clusters found following integration and clustering.
Annotation Method: manual

Ontology Term: mature natural killer cellCL:0000824
Synonyms Terms that have the same, or nearly the same, meaning as the selected cell type.: unknown
Category Class of which the cell type is a subclass.: natural killer cellCL:0000623
Ontology Assessment Disagreements with any aspect of the Cell Ontology.: —
Marker Gene Evidence Gene markers used by researcher to assign this cell annotation.: NCAM1, FCGR3A, KRLF1, NKG7, EOMES, KLRB1
Canonical Marker Genes: NCAM1, FCGR3A, EOMES
Rationale: This cell type was annotated as a natural killer cell by canonical gene expression, but displayed higher CD16 expression that the other natural killer cell subset within the dataset. Slightly lower transcript levels of NCAM1 may suggest ontology term CL:0000939 would be more appropriate however, flow cytometry staining did not indicate differing levels of CD56 at protein level. Standard accepted NK subsets, and described in tonsil Azimuth dataset
Rationale DOI: 10.1016/j.immuni.2024.01.006

Set of Labels Description: Cell annotations were based on the finest possible label that could describe the 16 clusters found following integration and clustering.
Annotation Method: manual