Human PBMC atlas - B memory cells

Summary

Metadata

Dataset Type

Integrated. The file includes an integrated collection of datasets.

Total Cells

337,659

organism

Homo sapiens

tissue

blood

assay

10x 3' v2 (102,181 cells)10x 5' v2 (95,087 cells)10x 5' v1 (89,113 cells)10x 3' v3 (51,278 cells)

disease

normal (184,830 cells)COVID-19 (116,309 cells)systemic lupus erythematosus (23,183 cells)infectious disease with sepsis (3,960 cells)respiratory system disorder (3,015 cells)post-COVID-19 disorder (2,267 cells)bacterial pneumonia (1,794 cells)infectious disease (1,492 cells)influenza (516 cells)viral pneumonia (293 cells)

Cell Labels

Level_4

Description

Clustering was Leiden based before manual annotation and consensus agreement by multiple authors.

Annotation Method

manual

Cell Labels (13)

Cells (337,659)

B IgA memory

46,468

B unswitched memory NR4A2

45,712

B type 2 IgE switched memory

43,809

B IgA switched memory ITGB1

41,531

B unswitched memory CR2

40,255

B unswitched memory

23,035

B IgA switched memory

20,068

B late activated / pre-memory

19,162

B atypical

17,647

B atypical CD27

16,386

B ISG switched memory

14,020

B late activated / early memory

7,601

Level_1

Description

Similar clusters with shared features from level 2 were manually merged to one level higher in the hierarchy to determine clustering.

Annotation Method

manual

Cell Labels (1)

Cells (337,659)

B_plasma

337,659

Level_2

Description

Similar clusters with shared features from level 3 were manually merged to one level higher in the hierarchy to determine clustering.

Annotation Method

manual

Cell Labels (1)

Cells (337,659)

B Memory/Effector

337,659

Level_3

Description

Similar clusters with shared features from level 4 were manually merged to one level higher in the hierarchy to determine clustering.

Annotation Method

manual

Cell Labels (2)

Cells (337,659)

B Memory

303,626

B atypical

34,033