Human PBMC atlas - B naive cells

LTB and BANK1 from NS-Forest are expressed in all of these B cell subsets but not in the plasma cell and plasmablast subsets

LTB and BANK1 from NS-Forest are expressed in all of these B cell subsets but not in the plasma cell and plasmablast subsets

(1) I'm a bit skeptical. If you check, this subpopulation is enriched in CD9. Given other relevant markers (e.g. CD24), wouldn't it be more convincing that these are a type of regulatory B cells? cf e.g. 10.3389/fimmu.2018.03034

(2) I don't really agree with adding VPREB3 as a marker here, as VRPEB3 is epxressed in all of the B cells presented (including the memory cells).

LTB and BANK1 from NS-Forest are expressed in all of these B cell subsets but not in the plasma cell and plasmablast subsets

VPreB has been debated in the past (https://doi.org/10.4049/jimmunol.161.3.1132) and found NOT to be expressed in mature peripheral B cells, possibly only in a subset of GC. This comment is: shall we keep biomarkers (RNAs) whose translation does not correspond to proteins? Should a note be made? This is a minefield, because there will be proteins (CD5 in B cells) for which RNA will not be detected.

Seems to be a typo here.
These are clearly not B plasma cells, as the rest of the annotations in this dataset are labeled as naive B cells.