Cross-species Spinal Cord Atlas: Cholinergic

• Gamma subtype:
  • Cacna1e
• Gamma*:
  • Ptprk

Cluster spans gamma and gamma* groups. Representative markers listed - not clear if actually expressed in these subtypes.

This is related to Trygve's comment on Cluster 140. Cluster 140 appears to contain mouse gamma and gamma* MNs. Cluster 140 is being annotated as "Alpha-MN" instead of being split and annotated as "Gamma_star_MN" and "Gamma-MN".

• Pitx2+ Cholinergic-IN:
  • Pitx2
• Gad1+ Cholinergic-IN:
  • Gad1

In mouse, we see Pitx2+ and Gad1+ subpopulations of cholinergic interneurons. This appears to be true in the NHP data as well! Cluster 262 corresponds to Pitx2+ Cholinergic-IN.

I agree with this annotation but wanted to mention that in mice, we see a subcluster of visceral MNs marked by Rxfp1. The Rxfp1+ subcluster corresponds to sacral visceral MNs while others correspond to thoracic visceral MNs. It does look like there is an Rxfp1+ subpopulation within the "Visceral Motor Neurons" group, but it appears to largely be from mouse cells.