This cell type was annotated as a natural killer cell by canonical gene expression, but displayed lower CD16 expression than the other natural killer cell subset within the dataset. Similar transcript levels of NCAM1 between two subsets also suggest that ontology term CL:0000938 would not be more appropriate in this case. Standard accepted NK subsets, and described in tonsil Azimuth dataset.
Cell annotations were based on the finest possible label to describe the 18 clusters found across both culture conditions (control and stimulated) following integration and clustering. Gene expression across clusters correlated with matched cell annotations in the uncultured, whole lymph node dataset (LN partial T and B cell depletion) in addition to some culture-specific cell clusters
