Summary
Non-inflamed human cystic lymph nodes were precision cut and cultured as tissue slices to enable ex vivo stimulation by a liposome-based adjuvant containing a synthetic toll-like receptor (TLR4) agonist and QS-21 saponin. Single-cell RNAseq datasets were integrated from control and adjuvant-stimulated conditions from six donors.
Metadata
- Dataset Type
- Integrated. The file includes an integrated collection of datasets.
- Total Cells
- 109,297
- organism
- Homo sapiens
- tissue
- cystic lymph node
- assay
- 10x 3' v3
- disease
- normal
Cell Labels
Description
Cell annotations were based on the finest possible label to describe the 18 clusters found across both culture conditions (control and stimulated) following integration and clustering. Gene expression across clusters correlated with matched cell annotations in the uncultured, whole lymph node dataset (LN partial T and B cell depletion) in addition to some culture-specific cell clusters
Annotation Method
manual
