Single-nucleus transcriptomic profiling of human skeletal muscle from healthy adults

Regenerating/Immature Myonuclei

Ontology Term

skeletal muscle fiberCL:0008002

Synonyms

Terms that have the same, or nearly the same, meaning as the selected cell type.

unknown

Category

Class of which the cell type is a subclass.

myotubeCL:0002372

Ontology Assessment

Disagreements with any aspect of the Cell Ontology.

—

Marker Gene Evidence

Gene markers used by researcher to assign this cell annotation.

TTN, CMYA5, ABLIM2, MALAT1, NEAT1, MLIP

Canonical Marker Genes

MLIP, MYBPC1, NEB, SVIL, TRDN, TTN, ACTA1, DES, TNNC1

Rationale

Regenerating/immature myonuclei are suggested by high levels of structural genes TTN, CMYA5, ABLIM2 together with nuclear RNAs MALAT1, NEAT1, PCNT, MLIP, and KIAA1217, with TTN and CMYA5 prominently upregulated relative to mature fibers.

Inherited from author_cell_type

Set of Labels Description

Cell annotations were generated with scType using marker genes curated from related publications, and then manually reviewed and adjusted based on the top highly variable genes in each Leiden cluster.

Annotation Method

both

Algorithm Name

scType

Algorithm Version

v1

Algorithm URL

https://github.com/IanevskiAleksandr/sc-type

• v1 Tissue-wide single-cell multiomic analysis of human gene regulation and cell diversity
• Single-nucleus transcriptomic profiling of human skeletal muscle from healthy adults