Human cells share an identical genome yet exhibit diverse functions shaped by their tissue environment and cell-type-specific regulatory programs. While single-cell transcriptomic atlases have mapped cellular diversity across many human tissues, comparable atlases of regulatory elements at single-cell resolution remain scarce, limiting our understanding of how regulatory codes vary across organs. To address this gap, we generated high-quality 10x Genomics Multiome profiles from 21 human tissues collected from 4 GTEx donors, jointly measuring gene expression and chromatin accessibility in over 450,000 single cells. This cross-organ resource enables systematic characterization of transcription factor activity, cis-regulatory elements, and their coupling to gene expression across diverse cell types and tissue contexts. Together, these data illuminate the regulatory diversity of human single cells across organs and provide a foundational reference for studying tissue-specific gene regulation in health and disease.
Skeletal muscle tissue was collected from four healthy adult donors and analyzed using the 10x Genomics Multiome platform. Unsupervised clustering identified 12 distinct clusters, which were annotated and consolidated into 11 cell types.
