Human skeletal muscle aging atlas
In this study we characterized the diversity of cell types in human skeletal muscle (intercostal muscle) across the lifespan (8 young donors ~20-40 yrs, 9 aged donors ~60-75 yrs) using single-cell and single-nuclei seiquencing totalling ~ 183, 000 cells. We leveraged the aforementioned dataset to systematically study change in cell type composition and gene expression between young and old adults, highlighting changes in the major skeletal muscle compartments, including muscle satellite cells, myofiber and muscle microenvironment including stromal, immune and vascular cell types. We identified decreased ribosome biogenesis in TNFRSF12A+ MuSCs, CCL2-driven inflammatory remodelling of the aged microenvironment, expansion of NMJ-accessory nuclei as a denervation response, and a slow-to-fast myonuclear shift plus MYH8+ regenerative myocytes compensating fast-twitch loss. Additionally, we generated a complementary mouse muscle aging dataset by profiling hindlimb muscles from young (n = 5, 3 months) and old mice (n = 3, 19 months), using single-cell and single-nucleus sequencing for comparison. Resource: https://www.muscleageingcellatlas.org/
Datasets (1)
- Human intercostal skeletal muscle aging atlas (scRNA-seq and snRNA-seq, young vs aged)183,161 cells